| Solution Information | help | |
| Enzyme: | Heat shock protein HSP 90-alpha | |
| inhibitor: | BDBM47997 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | Assay Overview: The purpose of this biochemical assay is to determine dose response curves for compounds identified as active in a set of previous experiments entitled, "Luminescence-based primary biochemical high throughput screening assay to identify inhibitors of the Heat Shock Protein 90 (HSP90)" (AID 1789), and that confirmed activity in a set of experiments entitled, "Luminescence-based confirmation biochemical high throughput screening assay for inhibitors of the Heat Shock Protein 90 (HSP90)" (AID 1846). This assay monitors the ability of compounds to inhibit the refolding of thermally denatured firefly luciferase by Hsp90 protein (7, 8). Rabbit reticulocyte lysate is the source of Hsp90 protein. In this assay, denatured luciferase enzyme is incubated with the cell lysate in the presence of test compounds. As designed, compounds that inhibit Hsp90 activity will prevent luciferase renaturation and activity, leading to a decrease in well luminescence. Compounds were tested in t | |
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